Hepatitis E Virus Infection in Macaca Mulatta

One of the five known hepatitis viruses that can infect humans is hepatitis E virus (HEV). The first documented infection that was caused by HEV occurred in 1955 during an outbreak in New Delhi, India (1). HEV is a small nonenveloped particle that belongs to the family Hep-eviridae and the genus Hepevirus. Its single-stranded RNA genome has approximately 7200 base pairs (2). By genomic sequence analysis, there are four genotypes (1-4) and at least 24 subgenotypes (1a-1e, 2a-2b, 3a-3j, and 4a-4g) of HEV in humans and other mammals (4). Avian isolates of HEV were initially proposed to constitute the fifth genotype of HEV; but, due to their shorter genome and low homology with mammalian isolates, they have been categorized as a member of a separate genus (3). HEV genotypes 1 and 2 are limited to humans, and genotypes 3 and 4 are common between humans and other mammalian species (5). Domestic animals, especially pigs, are the principal animal reservoirs for HEV (6), as are, to a lesser extent, boar, deer, rabbits, and rats (7, 8). In a study in China, a serum positivity test for anti-HEV antibodies was performed for farm animals. The highest positive rates of serum anti-HEV were detected in swine been used as animal models to study different aspects of HEV infection and for vaccine trials (10). Despite supporting HEV replication and producing antibodies against the virus, there are insufficient studies on the capacity of monkeys to serve as natural reservoirs for the virus. Huang et al. in a study on HEV seroepidemiology in China, found anti-HEV IgG and IgM in the serum of rhesus monkeys (Macaca mulatta) but not HEV RNA in their stool (11). Regarding the possibility of cross-species infection between wild animals or between human and animals, such as on farms, knowledge of the seroepidemiology of the virus and its natural reservoirs seems to be essential. Workers at pig farms and slaughterhouse workers have twice the level of anti-HEV compared with the general population (9). This may indicate the role of zoonotic infection by HEV. This is important , especially in xenotransplantations (12). Another study showed that in an area with both swine and Maca-ca mulatta, antibodies against HEV were present in both groups. However, despite the detection of swine HEV genotype 4 RNA in stool specimens, the same assay was negative for Macaca mulatta. Swine HEV can infect non-human primates; cross-species transmission of …